Characterization of Protein Kinase C Isotype Expression in Adult Rat Heart
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چکیده
The pattern of protein kinase C (PKC) isotype expression in whole extracts of dispersed, freshly isolated adult rat ventricular myocytes and adult rat heart ventricle was examined by immunoblot analysis using antisera specific for PKC-ea, -th, -, -6, -e, -g, or -q isotypes. This analysis revealed significant levels of expression of the Ca2+-independent isotype PKC-e, which was detected as a band of 97-kd molecular mass. PKC-gwas detected principally as a 66-kd band that probably represented a proteolytic product of the holoenzyme. PKC-ng was detected only in whole ventricle as a doublet at 75 and 81 kd and was therefore probably present in nonmyocytic cells. PKC-a, -I31, -y, and -6 could not be detected. Because of
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تاریخ انتشار 2005